作者：Rodríguez A, Shimada T, Cervera M, Alquézar B, Gadea J, Gómez-Cadenas A, de Ollas C, Rodrigo M.J, Zacarías L, Peña L.
作者单位：Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Spain
摘要：Volatile organic compounds, most of them being terpenes, are signals emitted by plants for communication with the environment. Orange fruit accumulates mainly terpenes in mature peel oil glands, D-limonene accounting for approximately 97% of terpene content. We have generated transgenic orange plants carrying a D-limonene synthase gene in antisense (AS) configuration. Transgenic expression caused a dramatic decrease in the accumulation of D-limonene in fruit peels, being about 80-100 times lower in AS samples than in empty vector (EV) transgenic ones. This affected the interaction of fruits with their biotic environment because they resulted to be resistant to different specialized pathogens. A gene expression analysis of these fruits linked the decrease of D-limonene to the upregulation of genes involved in the innate immunity response. Additionally, it caused an increase in the accumulation of jasmonates in AS orange peels, explaining their resistance to different fungi. Furthermore, this indicates for the first time an inverse correlation between volatile terpene accumulation upon fruit development and depletion of JA-mediated defense responses, thus stressing the importance of D-limonene for specialized pathogens to establish infections in mature orange peels. High D-limonene content in mature orange peels may be a signal for attractiveness of microorganisms which might be likely involved in facilitating the access to the pulp of seed dispersal frugivores.
文献注录：Rodríguez A, Shimada T, Cervera M, Alquézar B, Gadea J, Gómez-Cadenas A, de Ollas C, Rodrigo M.J, Zacarías L, Peña L.. Terpene downregulation triggers innate immunity and resistance to fungal pathogens in orange fruits [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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