作者：Alquézar B, Pons E, Rodríguez A, Rodrigo M.J, Zacarías L, Peña L.
作者单位：Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Spain
摘要：Orange fruit is an excellent natural dietary source of phytochemicals and nutrients (as vitamin C, carotenoids, flavonoids and folic acid, among others) contributing to its quality, nutritional value and health benefits. In recent years, considerable advances in the understanding of the molecular basis regulating carotenoid biosynthesis have been accomplished and biotechnological strategies to modify carotenoid content or composition in orange fruits are now feasible. For example, β-carotene (provitamin A) content in orange has been increased by blocking the expression of an endogenous β-carotene hydroxylase gene, involved in the conversion of β-carotene into xanthophylls. β-lycopene cyclase activity is a key metabolic step in the pathway as determinates a branching point for the accumulation of β,β-xanthophylls and may be also implicated in lycopene accumulation in some citrus species. Therefore, the chromoplast-specific gene is an excellent candidate for the metabolic engineering of carotenoid biosynthesis and its promoter may be also a good target to drive fruit-specific expression. Thus, with the aim of increasing β,β-carotenoid levels in orange tissues we are over-expressing β-LCY2 using its own promoter. Transgenic ‘Pineapple’ and ‘Valencia’ oranges carrying this construction are being generated. Detailed molecular and biochemical analysis of the transgenic lines will allow us to gain insights into carotenoid biosynthesis regulation in citrus.
文献注录：Alquézar B, Pons E, Rodríguez A, Rodrigo M.J, Zacarías L, Peña L.. Overexpression of a chromoplast-specific lycopene β-cyclase (β-LCY2) gene in orange fruit [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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