作者：Tavano ECR, Harakava R, Vieira MLC, Mourão Filho FAA, Mendes BMJ.
作者单位：Universidade de São Paulo (USP), Centro de Energia Nuclear na Agricultura, Brazil
摘要：Huanglongbing (HLB) associated with sieve tube-restricted bacteria Candidatus Liberibacter spp., is one of the most destructive disease affecting citrus. The aim of this study was to produce transgenic sweet orange plants (Citrus sinensis) expressing antimicrobial attacin A gene (attA) under the control of phloem tissue specific promoters, in order to obtain resistant plants to Candidatus Liberibacter spp. Genetic transformation of ‘Hamlin’ and ‘Valencia’ sweet orange cultivars was performed via Agrobacterium tumefaciens. The gene construct for genetic transformation included the attacin A gene controlled by either sucrose transporter gene (AtSuc2) or phloem protein 2 gene promoters (AtPP2) from Arabidopsis thaliana, or phloem protein 2 gene promoter (CsPP2) from C. sinensis. Epicotyls segments collected from in vitro germinated seedlings were used as explants for genetic transformation. Regenerated shoots were micrografted onto ‘Carrizo’ citrange seedlings for plant development. Transgenic plants were identified by PCR analyses. The plants were acclimatized and transferred to a greenhouse. Integration of the transgene was confirmed for ‘Hamlin’ and ‘Valencia’ cultivars containing the AtSuc2, the AtPP2 or the CsPP2 promoters controlling the expression of attA. The transgene transcription is been studied by northern blot or RT-qPCR analyses.
文献注录：Tavano ECR, Harakava R, Vieira MLC, Mourão Filho FAA, Mendes BMJ.. Genetic transformation of Citrus sinensis with attacin A gene driven by phloem tissue specific promoters for resistance to Candidatus Liberibacter spp [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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