作者：Soler N, Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña L.
作者单位：Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro de Protección Vegetal y Biotecnología, Spain
摘要：Citrus tristeza virus (CTV) is phloem-restricted in natural citrus hosts. The 23 kDa protein (p23) encoded by the virus is a RNA silencing suppressor and an important pathogenicity factor. Constitutive expression of p23 in transgenic citrus plants under the control of the 35S promoter incites, in addition to aberrations resembling CTV symptoms, others likely related to the ectopic expression of this protein in cells usually not infected by the virus. Moreover, transgenic expression of a fragment comprising only the N-terminal 157 amino acids (p23Δ158-209) elicit CTV-like leaf symptoms similar to, albeit milder than, those resulting from expression of the complete p23 protein. To discriminate between symptoms induced by p23 in CTV infection and other pleiotropic effects of this protein, we restricted expression of p23-derived transgenes to phloem-associated cells in ‘Mexican’ lime plants by means of the phloem-specific promoter from Commelina yellow mottle virus (CoYMV). The complete gene p23 from either the severe T36 or the mild T317 CTV isolates, or the fragment p23Δ158-209 from the former, were used. Expression of these constructs in the phloem incited CTV-specific symptoms like vein clearing in ‘Mexican’ lime leaves, but not the unspecific symptoms (epinasty, growth cease and apical necrosis) observed when p23 was expressed under the control of the 35S promoter. Moreover, vein necrosis was exclusively associated to phloem-specific expression of the transgenes derived from CTV T36.
文献注录：Soler N, Fagoaga C, López C, Moreno P, Navarro L, Flores R, Peña L. . Transgenic ‘Mexican’ lime plants expressing the gene p23 from Citrus tristeza virus (CTV) under the control of a phloem-specific promoter display symptoms closely similar to those accompanying virus i [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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