作者：Castro LM, Mendes BMJ, Mourão Filho FAA
作者单位：Universidade de São Paulo (USP), Departamento de Produção Vegetal, Brazil
摘要：The use of mature tissue for citrus genetic transformation is important for the precocious evaluation of the genetically modified characteristics. However, an efficient organogenesis protocol with high rates of plant regeneration is necessary. This work aimed to optimize a protocol for in vitro organogenesis of ‘Hamlin’, ‘Pera’, and ‘Valencia’ sweet orange cultivars (Citrus sinensis) using as explant internodal segments collected from adult plants cultured in greenhouse. Organogenesis was induced by culturing internodal segments in Petri dish (100 x 15 mm) contanining MS medium supplemented with sucrose (30 g∙L-1) and different concentrations of benzylaminopurine (BAP; 0.0, 1.0, 2.0, 3.0, and 4.0 mg∙L-1). The material was incubated in the dark at 27°C ± 2°C for 6 to 8 weeks. After adventitious bud development, the shoots were transferred to Petri dish (100 x 20 mm) containing MS medium with half concentration of BAP and incubated under 16-h photoperiod. The highest percentage of responsive explants was obtained with explants cultured in medium supplemented with 1 mg∙L-1 BAP. The explant responsiveness varied among cultivars. ‘Hamlin’ was the most responsive followed by ‘Valencia’ and ‘Pera’. Adventitious shoots were grafted ex vitro onto decapitated ‘Rangpur’ lime rootstocks (C. limonia). This protocol will be utilized for citrus mature tissue genetic transformation.
文献注录：Castro LM, Mendes BMJ, Mourão Filho FAA. Optimization of in vitro organogenesis and plantlet regeneration of sweet orange from mature tissue [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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