作者：Oliveri C, Distefano G, La Malfa S, La Rosa R, Deng Z, Gentile A.
作者单位：Dipartimento di Scienze delle Produzioni Agrarie e Alimentari (DISPA), Italy
摘要：Constitutive over-expression of antifungal genes from microorganisms involved in plant defence mechanisms represents an efficient strategy to confer genetic resistance against a broad range of phytopathogenic fungi. In lemon (Citrus limon), fungal diseases cause economic losses in the field as well as in postharvest management. In this study, fruits collected from two different transgenic lemon clones (SR23 and SR24) expressing a chitinase gene (chit42) from Trichoderma harzianum were tested in vivo to investigate the level of resistance against Penicillium digitatum, P. italicum, Colletotrichum gloesporioides and Botrytis cinerea. Fruits were artificially inoculated with conidial suspensions of the above mentioned fungi. Experiments were carried out in duplicated in climatic chamber and symptoms development was evaluated 6, 14 and 21 days after inoculation. As compared to control fruit, transgenic fruits did not show mould symptoms when inoculated with C. gloesporioides and B. cinerea during the whole evaluation period. A reduction in the lesion area of 50% was recorded after 6 days from inoculation with P. digitatum and P. italicum in transgenic fruits compared to the control. However, differences among the analyzed genotypes were not evident after the moulds. The analyzed transgenic lemon clones were obtained from a widespread appreciated lemon cultivar and did not show any difference in morphological and qualitative fruit traits compared to the control. Therefore, the results of this study suggested that their use could represent an alternative for sustainable management of lemon postharvest diseases.
文献注录：Oliveri C, Distefano G, La Malfa S, La Rosa R, Deng Z, Gentile A.. Lemon fruits from endochitinase transgenic plants exhibit resistance against postharvest fungal pathogens [A]. [C] Ⅶ International Citrus Congress. 2012.
报/刊名：《》，发表于2012
文献类型： [A]
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