作者：Petersen Y, Keremane M, Moore G, Niblett C, Lee R
摘要：Pokeweed antiviral protein (PAP) isolated from Phytolacca Americana has been shown to have a broad range antiviral activity. For in vivo experiments, the wild type PAP gene was cloned into a plant transformation vector. We also prepared a DNA construct which produced a deletion of 25 amino acids from the C-terminus of PAP, and two constructs with independent point mutations at the N-terminus (Gly75-Asp), and the active site (Glu176-Ala). These four constructs were then transformed into Citrus paradisi by Agrobacterium-mediated transfer. Nine transgenic plants containing the N-terminal mutant, one containing the active site mutation, and none with the other two constructs were obtained and characterized by molecular methods. The transgenic citrus plants are being graft- and aphid-challenged with CTV and will be analyzed for CTV replication.
文献注录：Petersen Y, Keremane M, Moore G, Niblett C, Lee R. Towards developing resistance to Citrus tristeza virus (CTV) by expressing using pokeweed antiviral protein in transgenic grapefruit [C]. Phytopathology. 2003, 06: S71.
报/刊名：《Phytopathology》，发表于2003 / 第 06 期。
文献类型： [C]
页码：第 S71 页 / 共1 页