作者：邱涛涛,王华,毛世红
作者单位：西南大学食品科学学院,中国农业科学院柑桔研究所
摘要：以石榴叶为原料,采用Folin-Ciocalteu法定量测定石榴叶提取液中总酚含量,通过单因素试验和正交试验对石榴叶总酚的提取工艺进行探讨。确定其最佳提取条件为:料液比1:25(g/ml),温度70℃,提取溶剂为75%乙醇,提取时间1.5h。综合考虑成本因素,原料过1mm筛较为适宜,按照该工艺提取石榴叶总酚的得率为16.63%。
关键词：石榴叶,总酚,Folin-Ciocalteu法,提取
Title: Study on Determination and Extraction of Total Polyphenols from Leaves of Punica granatum L.
Authors: QIU Tao-tao,WANG Hua,MAO shi-hong
Abstract: Folin-Ciocalteu method was used to determine the content of total polyphenols from leaves of Punica granatum L.. The effect of extraction conditions on the extraction rate of total polyphenols from extracts was evaluated by single factor and orthogonal tests. The optimal extraction conditions of total polyphenols from leaves of Punica granatum L. are as follows;75% alcohol as extraction solvent, temperature 70 ℃, extraction time1.5 h, material to liquid ratio 1:20 and 1 mm of particle size. Under these conditions, the maximum extraction rate of total polyphenols from leaves of Punica granatum L. was 16.63%.
Key words: leaves of Punica granatum L.； total polyphenols； Folin-Ciocalteu； extraction；
文献注录：邱涛涛,王华,毛世红. 石榴叶总酚测定及提取工艺研究 [J]. 食品科学. 2009, 10: 131-134.
报/刊名：《食品科学》，发表于2009 / 第 10 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 131-134 页 / 共4 页