作者：周映霞,张丽杰,潘家荣,焦必宁,王磊
作者单位：西南大学食品科学学院,中国农业科学院农产品加工研究所,中国农业科学院柑桔研究所,西南大学柑桔研究所
摘要：以咪唑乙醇为原料,与6-溴己酸乙酯发生取代反应后再经水解反应合成抑霉唑半抗原(6-[1-(2,4-二氯苯基)-2-(1-咪唑基)乙氧基]己酸)。产物经薄层层析和1H-NMR鉴定后,采用活化酯法分别与牛血清蛋白(BSA)和卵清白蛋白(OVA)偶联得到免疫原(HIA-BSA)和包被原(HIA-OVA),紫外光谱分析法计算得其偶联比分别为13:1和7:1,初步说明人工抗原合成成功。通过免疫动物获得效价为1:64000的抗体,采用间接竞争ELISA法测定抗体的IC50值为1.76μg/mL,从而进一步证明人工抗原合成成功,所得的抗体可用于ELISA检测试剂盒的研制。
关键词：抑霉唑,半抗原,人工抗原,合成,鉴定
Title: Synthesis and Identification of Imazalil Antigen
Authors: ZHOU Ying-xia,ZHANG Li-jie,PAN Jia-rong,JIAO Bi-ning,WANG Lei
Abstract: In this study, imazalil (6-[1-(2,4-dichloro-phenyl)-2-(1-imidazolyl) ethoxy] hexanoic acid) was synthesized via substitution reaction between imidazole ethanol and 6-bromo-ethyl followed by hydrolysis reaction to provide a hapten. The synthesized product was identified by thin-layer chromatography (TLC) and 1H-NMR. The hapten was conjugated with bovine serum albumin (BSA) and ovalbumin (OVA) to obtain artificial immune antigen and artificial coating antigen, respectively. The coupling molar ratios were calculated to be 13:1 and 7:1 according to UV spectroscopy analysis, which suggests that artificial antigens are both synthesized successfully. The titer of the antibodies secreted by immunized animals was 1:64000 and the IC50 value was 1.76μ g/L determined by indirect ELISA, which further confirms successful synthesis of artificial antigens. The obtained antibodies can be used for the development of ELISA test kit
Key words: imazalil； hapten； antigen； synthesis； identification；
文献注录：周映霞,张丽杰,潘家荣,焦必宁,王磊. 杀菌剂抑霉唑人工抗原的合成和鉴定 [J]. 食品科学. 2010, 13: 184-188.
基金: “十一五”国家科技支撑计划项目(2007BAD47B07);国家“863”计划项目(2006AA10Z449);国家现代农业(柑橘)技术创新体系项目
报/刊名：《食品科学》，发表于2010 / 第 13 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 184-188 页 / 共5 页