作者：胡军华,马丽娜,冉春,李鸿筠,姚廷山,刘浩强,雷慧德
作者单位：中国农业科学院柑桔研究所,柑桔学重庆市市级重点实验室,西南大学植物保护学院
摘要：【目的】探讨杀螨植物牛蒡Arctium lappaL.提取物中主要杀螨成分L1-2的杀螨作用机理。【方法】采用叶片浸渍法处理桔全爪螨Panonychus citri雌成螨后,测定了静止期、兴奋期、痉挛期、麻痹期、复苏和死亡期5个中毒阶段试虫体内几种代谢酶的活性。【结果】L1-2组分在静止期和复苏期对羧酸酯酶(carboxylesterase,CarE)具有一定的抑制作用,在其他时期均激活CarE活性。除了静止期外,在其他时期均能激活乙酰胆碱酯酶(acetylcholinesterase,AChE)和谷胱甘肽转移酶(glutathione S-transferases,GSTs)的活性,在痉挛期和麻痹期活性增强,随后在麻痹期和复苏期降低。【结论】L1-2组分对CarE的抑制与其毒杀活性有关,而中毒试虫的复苏可能与AChE和GSTs有关。该组分可在较长时期内影响桔全爪螨的神经传导及消化和生殖系统,具有潜在的应用研究价值。
关键词：桔全爪螨,牛蒡,杀螨机理,代谢酶,杀螨活性
Title: Toxicity of L1-2 fraction of Arctium lappa against Panonychus citri(McGregor)(Acari:Tetranychidae)and its effects on several metabolic enzymes
Authors: HU Jun-Hua,MA Li-Na,RAN Chun,LI Hong-Jun,YAO Ting-Shan,LIU Hao-Qiang,LEI Hui-De
Abstract: [Objective] To study the acaricidal mechanism of the fraction L1-2,the main acaricidal component in the extracts of Arctium lappa L.[Methods]The female Panonychus citri(McGregor)adults were treated by the fraction L1-2 with contact application.The activities of carboxylesterase(CarE),acetylcholine esterase(AChE)and glutathione S-transferases(GSTs)in mites were determined at all poisoning stages.[Results]The CarE activity was inhibited at the stillness stage and the recovery stage,but was induced at the other stages.The activities of AChE and GSTs were induced at all poisoning stages but the stillness stage,and strongly enhanced at the convulsion stage and the paralysis stage,then gradually recovered at the paralysis stage and the recovery stage.[Conclusions]The CarE activity is related with toxicity of the fraction L1-2 against the female P.citri adults,but the recovery of poisoned mites is probably due to the GSTs and AChE activities induced.L1-2 fraction can affect not only the nerve transition but also the digestive and reproductive system of mites in a long time,so it has potential value in acaricidal research and development.
文献注录：胡军华,马丽娜,冉春,李鸿筠,姚廷山,刘浩强,雷慧德. 牛蒡L1-2组分对桔全爪螨的毒性和几种代谢酶的作用 [J]. 昆虫学报. 2010, 04: 405-410.
基金: 公益基金项目(nyzx07-057);国家科技支撑计划项目(2008BAD92B08-5-5)
报/刊名：《昆虫学报》，发表于2010 / 第 04 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 405-410 页 / 共6 页