作者：王福生,江东*
作者单位：西南大学园艺园林学院,中国农业科学院柑桔研究所
摘要：将叶绿体SSR和EST-SSR标记用于揭示柑橘及其近缘属的系统发育关系,并阐明保存在重庆国家果树种质柑橘圃内一些特异种质资源的遗传背景。其中50份材料和44份材料分别用cpSSR和EST-SSR分析。22对cpSSR引物产生146个多态性条带,平均检测到6.64个多态性条带,平均Shannon's多样性指数为0.32;18对EST-SSR引物产生240个多态性条带,平均检测到13.3个多态性条带,平均多态信息含量(PIC)为0.73,平均期望杂合度为0.78。根据cpSSR和EST-SSR分析,结合形态学证据,阐明一些特异柑橘种质资源的遗传背景,同时证明莽山野橘、元橘、宜昌橙有较近的亲缘关系。
关键词：柑橘,cpSSR,EST-SSR,遗传背景
Title: Studies on Genetic Background of Important Germplasm Resources Among Citrus Based on cpSSR and EST-SSR Marker
Authors: WANG Fu-sheng,JIANG Dong
Abstract: Chloroplast simple sequence repeat(cpSSR)and expressed sequence tag-simple sequence repeat(EST-SSR)markers were used to reveal Citrus and its related genera phylogenetic relationship and to clarify the genetic background of some unique citrus germplasms conservered in National Citrus Germplasm Repository(Chongqing).Fifty and forty-four accessions were used for cpSSR and EST-SSR analysis respectively.Twenty-two pairs of cpSSR specific primers generated 146 bands with an average of 6.64 alleles per locus and an average Shannon’s information index of 0.32;Eighteen pairs of EST-SSR primers produced 240 bands with the mean of 13.3 alleles per locus,the mean polymorphic information content(PIC)was 0.73 and expected heterozygosity was 0.78.In addition,the genetic origin of some unique citrus accessions were also clearly clarified by cpSSR and EST-SSR analysis combining with the morphological characteristics,meanwhile,it was showed that Ichang Papeda have close phylogenetic relationship with Mangshan Yeju and Yuan Ju.
Key words: Citrus； cpSSR； EST-SSR； genetic background；
文献注录：王福生,江东*. 应用cpSSR和EST-SSR标记进行柑橘特异种质资源遗传背景研究 [J]. 园艺学报. 2010, 03: 465-474.
基金: ‘十一五’国家科技支撑计划项目(2006BAD13B06-1-4);国家自然科学基金项目(30671450);市自然科学基金项目(CSTC,2007BB1343)
报/刊名：《园艺学报》，发表于2010 / 第 03 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 465-474 页 / 共10 页