作者：彭祝春,龚桂芝,陈善春,张戈壁,洪棋斌
作者单位：中国农业科学院柑桔研究所,国家柑桔工程技术中心,广西柑桔研究所
摘要：以抗柑橘溃疡病的‘宜昌橙2586’与易感病的‘岭南沙田柚’进行杂交,随机选取F1代材料80株,开展溃疡病抗性田间接种鉴定。鉴定结果表明,溃疡病抗性在F1代分离明显,若将免疫和高抗类型均视为抗病,中抗、中感和感病类型均视为感病,则抗感的分离比例接近1︰1。采用集合分离分析法BSA(Bulked Segregant Analysis),结合SSR分子标记技术,筛选到1个与柑橘溃疡病抗性相关的分子标记CCR-110。利用F1代分离个体和公认的抗感材料对标记进行验证分析,发现该标记表现较好的抗性相关性。F1群体相关分析表明,该标记与柑橘溃疡病抗性的相关系数为0.79;采用最大似然法计算重组率为9.38%±3.5%,通过Kosambi函数将其转换成图距单位(cM),遗传距离为9.11cM;感病的甜橙类、柚类、枳类没有此标记,抗病的金柑类、宜昌橙类、香橙类、宽皮柑橘类的多数材料出现此标记。
关键词：柑橘,柑橘溃疡病,抗性鉴定,分子标记
Title: 柑橘溃疡病抗性相关的SSR标记筛选 Screening of Molecular Marker Putatively Related with Citrus Canker Resistance
Authors: PENG Zhu-chun,GONG Gui-zhi,CHEN Shan-chun,ZHANG Ge-bi,and HONG Qi-bin
Abstract: Eighty plants from the F1 population of Ichang Papeda 2586(resistant genotype)×Lingnan Shatian Pummelo(susceptible genotype) were inoculated with citrus canker [Xanthomonas axonopodis pv.citri(Xac)] for resistance evaluation in field.The results revealed that resistance segregation in the F1 progenies was significant,and the ratio of resistant and susceptible plants was close to 1:1.If immune and highly resistant types were regarded as disease resistance and moderate resistance,moderate susceptible and susceptible were regarded as susceptible types.A SSR molecular marker CCR-110 putatively related with resistance of citrus canker was obtained based on BSA(Bulked Segregant Analysis).Validation analysis of the marker was performed using F1 segregation population tested and resistant and susceptible genotypes generally known.Marker CCR-110 was found to show good resistance relevancy.Co-segregation analysis showed that correlation coefficient between the marker and citrus canker resistance was 0.79.The recombination frequency was calculated with maximum likelihood method and transformed into centimorgans(cM) according to the Kosambi function.The recombination frequency is 9.38%±3.5% and genetic distance is 9.11 cM.The marker was not found in susceptible genotypes(sweet orange,pummelo and trifoliate orange),but it was observed in most resistant ones(Kumquat,Ichang Papeda,Yuzu,and mandarin).
Key words: Citrus; citrus canker; resistance test; molecular marker
文献注录：彭祝春,龚桂芝,陈善春,张戈壁,洪棋斌. 柑橘溃疡病抗性相关的SSR标记筛选 [J]. 园艺学报. 2010, 03: 383-389.
基金: 国家科技支撑计划项目(2008BAD92B05);国家‘863’计划项目(2006AA100108);市‘十一五’柑桔选引种重大专项(2007-2010)
报/刊名：《园艺学报》，发表于2010 / 第 03 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 383-389 页 / 共7 页