作者：邹敏,宋震,唐科志,董鹏,周常勇*
作者单位：中国农业科学院柑桔研究所,西南农业大学植物保护学院
摘要：对比分析了3种黄龙病病原DNA微量提取方法,方法1和方法2分别采用Sandrine等人和Hung等人的黄龙病病原DNA提取方法,但取样量分别由100mg和250mg减少为20mg和10mg;方法3采用周常勇等人的微量核酸提取方法,取样量为10mg。实验结果表明方法1提取的病原DNA浓度低、杂质多,PCR效果差;方法2提取的病原DNA浓度较高、杂质少,PCR效果好,但提取周期较长,不适合大批量样品的PCR快速检测;方法3提取的病原DNA浓度较高、杂质少,PCR效果好,且提取速度快,适宜大批量样品的PCR快速检测。
关键词：柑橘黄龙病病原,微量提取,DNA
Title: Comparing of micro extraction methods of DNA from citrus huanglongbing pathogen
Authors: Zou Min,Song Zhen,Tang Ke-zhi,Dong Peng,Zhou Chang-yong
Abstract: Three methods of microextractionof DNAfromcitrus huanglongbing pathogen has beencom-pared. Method 1 and method 2 are followedthe methods conducted by Sandrine et al .and Hung et al .,but the amount of the sample is reducedfrom100mg and 250mgto 20mg and 10mgrespectively. Method3 is followedthe method conducted by Zhou Changyong et al .,the amount of the sample is 10mg.Theresult showsthatthe product of method 1 containslowconcentrationof DNAand muchimpurity,itis un-fit for PCRdetectionof huanglongbing;product of method 2 contains highconcentrationof DNAandlittleimpurity,it isfit for PCRdetection of huanglongbing;product of method 3 contains highconcentration ofDNAandlittleimpurity,it is alsofit for PCRdetectionof huanglongbing.Sincethe progressfor extractionin method 3 is quicker thanthat of method 2 ,method 3 is more fit for PCRdetection of a lot of samples .
Key words: citrus huanglongbing pathogen； micro extraction； DNA；
文献注录：邹敏,宋震,唐科志,董鹏,周常勇*. 柑橘黄龙病病原DNA微量提取方法比较 [J]. 植物检疫. 2005, 05: 271-274.
报/刊名：《植物检疫》，发表于2005 / 第 05 期。
文献类型： [J]
页码：第 271-274 页 / 共4 页