作者：周彦,周常勇,李中安,王雪峰,刘科宏
作者单位：西南大学柑桔研究所,中国农业科学院柑桔研究所
摘要：【目的】获得对中国甜橙茎陷点型衰退病具有防治作用的柑桔衰退病毒(Citrus tristeza virus,CTV)弱毒株。【方法】对7个国内外CTV弱毒分离株进行褐色桔蚜接种强毒株的拮抗试验,并运用p25/HinfⅠRFLP组群分析、特异性RT-PCR分析和寄主植株症状观察的方法对拮抗试验效果进行检测。【结果】本研究中所用7个弱毒株对甜橙茎陷点型衰退病均有一定的防治作用,其中弱毒株CT9、CT17和CT77对茎陷点型强毒株CT14的拮抗效果最为明显,分别为70%,50%和40%。分子检测与生物学检测的结果相一致。【结论】初步筛选出的具有拮抗作用的CTV弱毒株CT9、CT17和CT77具有应用于交叉保护的潜力,可供进一步田间区试研究。
关键词：柑桔衰退病毒,交叉保护,防效检测
Title: Mild Strains Cross Protection Against Stem-Pitting Tristeza of Sweet Orange
Authors: ZHOU Yan,ZHOU Chang-yong,LI Zhong-an,WANG Xue-feng,LIU Ke-hong
Abstract: This study was conducted to obtain the protective mild isolates of Citrus tristeza virus(CTV) to protect sweet orange plants against super-infection of the severe stem-pitting CTV isolates.【Method】Sweet orange seedlings were graft-inoculated with seven mild CTV isolates and challenge-inoculated with severe stem-pitting isolates born by brown citrus aphids(Toxoptera citricida Kirkaldy).The effectiveness of the cross protection was evaluated by p25/Hinf ⅠRFLP groups assay,special RT-PCR assay and stem-pitting examination.【Result】Seven CTV mild isolates applied in this study were all experimentally proven to have somehow protective effectiveness against severe stem-pitting CTV isolates,of which mild isolates of CTV CT9,CT17 and CT77 had more significant effectiveness than the others.【Conclusion】The mild CTV isolates CT9,CT17 and CT77 have a potential usage in mild strain cross protection(MSCP),and further field challenge trials are needed.
Key words: Citrus tristeza virus； Cross protection； Detection of effectiveness；
文献注录：周彦,周常勇,李中安,王雪峰,刘科宏. 利用弱毒株交叉保护技术防治甜橙茎陷点型衰退病 [J]. 中国农业科学. 2008, 12: 4085-4091.
基金: 国家自然科学基金项目(30471205,30771485);科技部社会公益专项(nyhyzx07-023);市重点攻关项目(2005AB1010)
报/刊名：《中国农业科学》，发表于2008 / 第 12 期。
文献类型： [J]
页码：第 4085-4091 页 / 共7 页