作者：易时来,邓烈,何绍兰,郑永强,张璇
作者单位：西南大学柑桔研究所,中国农业科学院柑桔研究所,国家柑桔工程技术研究中心
摘要：以单系枳砧蓬安100号锦橙为试材,采用田间试验的方法开展利用鲜叶可见近红外光谱估测叶片氮素营养状况的研究。运用偏最小二乘法(PLS)分析叶片反射光谱与氮含量之间的关系。结果表明,在可见光350～700nm,随着氮肥用量的增加,叶片光谱反射率呈下降趋势;而在750～1075nm范围内,叶片光谱反射率随着氮肥用量增加而增加。通过对叶片反射光谱、一阶微分、二阶微分和倒数对数光谱进行变量标准化(SNV)处理,运用偏最小二乘法(PLS)与内部交叉验证建立的氮含量预测回归模型,其中反射光谱的一阶微分光谱氮含量定标模型具有最好的预测能力,其建模与预测均方根误差和标准差都较低且接近,偏差绝对值最小。因此,可以利用叶片反射光谱的一阶微分值来预测蓬安100号锦橙叶片氮含量。
关键词：锦橙,叶片,氮含量,偏最小二乘法,可见近红外光谱
Title: Research on nitrogen content of leaf of Jincheng orange cultivar using visible near infrared spectroscopy model
Authors: YI Shi-lai,DENG Lie,HE Shao-lan,ZHENG Yong-qiang,ZHANG Xuan
Abstract: Visible/near-infrared spectroscopy (Vis/NIRS) appears as a prominent technique for non-destructive evaluation. In this research,the potential of using the Vis/NIRS was investigated for measuring the nitrogen content in Peng’an 100 Jincheng orange leaves,and the relationship was established between non-destructive VNIRS measurement and the nitrogen content in Peng’an 100 Jincheng orange leaves. Intact Peng’an 100 Jincheng orange leaves were measured by reflectance VNIR in 350 to 1000 nm range. The data set as the reflectance VNIR was analyzed in order to build the best prediction model for these characteristic,using several spectral pretreatments such as First Derivatives Spectrum (FDS),Second Derivatives Spectrum (SDS) and Reciprocal Logarithm Spectrum [Log (1/R)] with Standardization of Variables (SNV) techniques. The results showed the reflectance spectrums of leaves presented downward trend within 350 to 700 nm and upward trend within 750 to 1000 nm with the increasing of the nitrogen fertilizer in potted plant of Peng’an 100 Jincheng orange (Citrus sinensis Osbeck.) on trifoliate (Poncirous trifoliata) rootstock. The model for the nitrogen content in Peng’an 100 Jincheng orange leaves prediction using FDS with SNV spectral pretreatments showed an excellent prediction performance. This non-destruc-tive,fast and accurate technology can be used in citrus industry that would be beneficial to predict the plant nutrition.
文献注录：易时来,邓烈,何绍兰,郑永强,张璇. 锦橙叶片氮含量可见近红外光谱模型研究 [J]. 果树学报. 2010, 01: 13-17.
基金: 重庆市重大攻关项目(CSTC,2006);科技部支撑计划项目(2007BAD47B04-1,2008BAD92B08-7-4);市柑桔学重点实验室和重庆市教委果树学重点实验室联合资助
报/刊名：《果树学报》，发表于2010 / 第 01 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 13-17 页 / 共5 页