作者：青玲,牛颜冰,吴楚钊,肖崇刚,周常勇*
作者单位：西南大学植物保护学院,植物病害生物学重庆市高校级重点实验室,中国农业科学院柑桔研究所,山西农业大学,饶平出入境检验检疫局
摘要：为明确主要烟区烟草病毒病的主要病毒种类,采用抗原直接包被ELISA法对重庆、四川、云南、贵州、河南、陕西和甘肃等省市部分烟区采集的266个烟草病毒病样品进行了检测。结果表明,样品中共检测到马铃薯Y病毒(Potato virus Y,PVY)、烟草花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)及芜菁花叶病毒(Turnipmo-saic virus,TuMV)等5种病毒。其中PVY的侵染最普遍,总检出率达75.56%;TMV、CMV、ToMV和TuMV的总检出率分别为65.41%、57.14%、36.84%和34.59%,其中,ToMV和TuMV仅在云南、四川、贵州和重庆样品中检测到。在257个阳性样品中有97个样品受CMV、TMV或PVY单独侵染,其检出率分别为7.39%、13.23%和17.12%;其余样品均受2种以上病毒复合侵染,其中55.63%的样品中检测到5种病毒复合侵染。田间烟草样品表现出复杂的病毒病症状类型。这些结果说明田间烟草病毒复合侵染现象严重,PVY已成为当前我国烟区的优势毒原。
关键词：烟草,病毒,复合侵染
Title: Virus Detection of Tobacco Viral Disease and Analysis of Viruses Mixed-infection in Some Tobacco Growing Areas
Authors: QING Ling,NIU Yan-bing,WU Chu-zhao,XIAO Chong-Gang,ZHOU Chang-yong
Abstract: In order to investigate the main viruses causing tobacco viral diseases in main tobacco-growing areas,266 tobacco leaf samples with viral disease symptoms collected from part of the tobacco-growing areas in Chongqing,Sichuan,Yunnan,Guizhou,Henan,Sha’anxi and Gansu were tested by antigen direct coated enzyme-linked immunosorbent assay(ELISA).Tobacco mosaic virus(TMV),Cucumber mosaic virus(CMV),Tomato mosaic virus(ToMV),Turnip mosaic virus(TuMV)and Potato virus Y(PVY)were detected.The infection percentage(75.56%)of PVY was the highest,and that of TMV,CMV,ToMV and TuMV was 65.41%,57.14%,36.84% and 34.59%,respectively.ToMV and TuMV were detected only in the samples from Yunnan,Sichuan,Guizhou and Chongqing.Of the 257 positive samples,97 cases were infected by CMV(7.39%),TMV(13.23%)or PVY(17.12%)alone.Other positive samples were infected by two or more viruses and about 55.63% of them were infected by all the five viruses.Tobacco samples in the field showed complex viral disease symptoms.These results indicated that viral mixed-infection occurred seriously in field tobacco and PVY has become a dominant virus in some tobacco-growing areas.
Key words: Tobacco； Virus； Mixed-infection；
文献注录：青玲,牛颜冰,吴楚钊,肖崇刚,周常勇*. 部分烟区烟草病毒病病原检测及复合侵染分析 [J]. 烟草科技. 2009, 12: 58-64.
基金: 国家青年科学基金项目“烟草曲茎病毒病害复合体种群遗传结构及变异研究”(30700524);西南大学博士后基金资助项目“我国部分地区烟草和蔬菜病毒病主要病毒种类的检测及鉴定”(207001)
报/刊名：《烟草科技》，发表于2009 / 第 12 期。
文献类型： [J]
页码：第 58-64 页 / 共7 页