作者：青玲,包凌云,周常勇,杨水英,孙现超
作者单位：西南大学植物保护学院,中国农业科学院柑桔研究所
摘要：利用7种植物病毒的抗体,对采自重庆7个区县的67份南瓜病毒病样品进行了抗原直接包被酶联免疫吸附分析(ACP-ELISA)检测。检测结果表明,在这67份样品中,至少59份感染了所要检测病毒,其中,感染黄瓜花叶病毒(Cucumber mosaic virus,CMV)、烟草花叶病毒(Tobacco mosaic virus,TMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)、芜菁花叶病毒(Turnip mosaic virus,TuMV)、蚕豆萎蔫病毒2号(Broad bean wilt virus 2,BBWV-2)、马铃薯Y病毒(Potato virus Y,PVY)和马铃薯X病毒(Potato virus X,PVX)的样品分别为80.60%、77.61%、74.63%、82.09%、76.12%、83.58%和55.22%。在检测呈阳性的59份样品中有93.22%的样品受到2种以上病毒的复合侵染,而受7种病毒复合侵染的样品高达60.00%,表明在南瓜上病毒复合侵染现象相当严重。进一步对54份受CMV侵染的南瓜样品检测发现,CMV亚组Ⅰ(CMVⅠ)病毒侵染的样品有49份,占90.74%,CMV亚组Ⅱ(CMVⅡ)病毒侵染的样品为11份,占20.37%;其中6份(11.11%)样品检测到CMVⅠ和CMVⅡ的复合侵染。以免疫捕获反转录PCR(IC-RT-PCR)扩增了一个CMVⅠ分离物(CMV-CQ)近全长CP基因并进行序列分析,结果证实其属于CMV亚组Ⅰ型,其核苷酸序列与国内CMVⅠ型赤豆分离物(CMV-RB)的同源性最高,为98.1%。
关键词：南瓜病毒病,ELISA检测,黄瓜花叶病毒亚组,外壳蛋白基因,序列变异
Title: ELISA Detection of Infectious Pathogenic Viruses and Variation of CMV from Squash in Chongqing
Authors: QING Ling,BAO Ling-yun,ZHOU Chang-yong,YANG Shui-ying,SUN Xian-chao
Abstract: The 67 squash samples with viral symptoms collected from seven squash-growing counties in Chongqing were detected by antigen–coated plate(ACP)-ELISA with antibodies against seven plant viruses.The results showed that at least 59 samples with in these 67 squash samples were infected by the viruses,of which 80.60%、77.61%、70.15%、82.09%、76.12%、83.58% and 55.22% of the samples were infected by Cucumber mosaic virus(CMV),Tobacco mosaic virus(TMV),Tomato mosaic virus(ToMV),Turnip mosaic virus(TuMV),Broad bean wilt virus 2(BBWV-2),Potato virus Y(PVY) and Potato virus X(PVX),respectively.Among 59 positive samples,93.22% of the samples were mix-infected with more than two viruses,and about 60% samples were mix-infected by the above seven viruses,indicating the mix-infections of viruses on squash were severely.Moreover,the results showed that among 54 samples infected by CMV,49(90.74%)samples were infected by CMV subgroupⅠ(CMVⅠ)and 12(22.22%)samples by CMV subgroup.Comparison of the nucleotide sequences showed that it has the highest identity(98.1%) with the previously reported CMVⅠisolate infecting Phaseolus angularis(CMV-RB)from China.
文献注录：青玲,包凌云,周常勇,杨水英,孙现超. 重庆南瓜病毒病病原ELISA检测及CMV变异分析 [J]. 园艺学报. 2010, 03: 405-412.
基金: 科技部科技基础性工作专项(2006FY111000);西南大学博士后基金项目(207001)
报/刊名：《园艺学报》，发表于2010 / 第 03 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 405-412 页 / 共8 页