作者:许兰珍,何永睿,姜国金,刘小丰,陈善春*
作者单位:中国农业科学院柑桔研究所,国家柑桔品种改良中心,西南大学园艺园林学院
摘要:cDNA文库是指生物不同生长发育时期特定组织或器官所转录的全部mRNA经反转录形成的cDNA与载体连接后形成的克隆的集合,cDNA文库是在基因组水平上研究某一生物特定器官、组织和发育时期基因表达的前提和基础。笔者就近年来cDNA文库的构建及其在植物抗性研究中的应用作一综述。
关键词:cDNA文库,植物抗性,基因克隆,基因表达,EST
Title: Construction of cDNA Library and its Application in the Study on Plant Resistance
Authors: XU Lan-zhen,HE Yong-rui,JIANG Guo-jin,LIU Xiao-feng,CHEN Shan-chun
Abstract: cDNA library is the pool of cDNA fragments which are ligated with vectors,and cDNA fragments are reverse-transferred from total mRNAs of some organism at certain period.cDNA library has the important significance in the research on the differential gene expressing of different tissues and growth-periods.In the paper the kind of cDNA library construction was simply summarized and the application of cDNA library in the study on plant resistance was described. 更多还原
Key words: cDNA library; Plant resistance; Gene cloning; Gene express; EST;
文献注录:许兰珍,何永睿,姜国金,刘小丰,陈善春*. cDNA文库构建及其在植物抗性研究中的应用 [J]. 安徽农业科学. 2007, 03: 660-662+664.
基金: 科技部“973”重大基础研究前期研究专项基金(2005CCA02900);市自然科学基金(SCTC2005BA1010)
报/刊名:《安徽农业科学》,发表于2007 / 第 03 期。
文献类型: [J]
页码:第 660-662+664 页 / 共4 页