作者：吕婵娟,周常勇,周彦,唐科志,李小松
作者单位：西南大学植物保护学院,中国农业科学院柑桔研究所
摘要：柑橘鳞皮病是一种极具破坏性的柑橘病害,广泛分布于南美及地中海地区。我国目前缺乏对该病害的快速检测技术。以柑橘鳞皮病毒(CPV)的外壳蛋白基因(CPG)为靶标,初步建立了一步法RT-PCR快速检测体系,扩增产物与NCBI数据库中已知CPVCPG的核苷酸序列进行比对,2者序列间同源性超过99%。
关键词：柑橘鳞皮病毒,一步法RT-PCR,检测
Title: Detection of Citrus psorosis virus by one-step RT-PCR and its annual distribution in different parts of Dweet tangor
Authors: Lü Chan-juan,Zhou Chang-yong,Zhou Yan,Tang Ke-zhi
Abstract: From May 2005 to April 2006,annual distribution of Citrus psorosis virus(CPV) on the leaves and shoots of Dweet tangor seedlings was detected by using one-step RT-PCR once a month.CPV was constantly detected in all leaves and barks of the infected plants in greenhouses and in old leaves and barks of the infected plants in screenhouses all the year round.Young leaves and barks of both summer and autumn shoots expect some summer shoots in screenhouses were consistently detected positive for CPV.These results suggested that the most suitable parts of citrus plant for sampling for diagnosis by one-step RT-PCR are old leaves and barks.
Key words: Citrus psorosis virus(CPV)； one-step RT-PCR； annual distribution；
文献注录：吕婵娟,周常勇,周彦,唐科志,李小松. 一步法RT-PCR检测柑橘鳞皮病毒 [J]. 果树学报. 2007, 01: 113-114.
基金: 农业部“948”后续项目(2004-C16-1);科技部社会公益专项(2005DIA4J053)。
报/刊名：《果树学报》，发表于2007 / 第 01 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 113-114 页 / 共2 页