作者：李勤,盛占武,孙志高*
作者单位：四川工商职业技术学院,西南大学食品学院,中国农业科学院柑桔研究所
摘要：实时荧光定量PCR技术是通过检测PCR产物中荧光讯号强度来达到定量的目的,不仅实现了对核酸信息量的分析比较,而且与常规PCR相比,它具有特异性更强、能有效解决PCR污染问题、自动化程度高等特点,近年来该技术开始作为食品微生物的研究手段。本文概述了实时荧光定量PCR技术的原理、优缺点及其在食品微生物检测中的应用与研究进展,并探讨了它的技术发展和应用前景。
关键词：实时荧光定量PCR,食品微生物,定量检测
Title: Application of Real-time Fluorescent Quantitative PCR for Detection and Resarch Microbes in Foods
Authors: LI QIN, SHENG ZHan-wu, SUN ZHi-gao
Abstract: Real-time fluorescent quantitative PCR(FQ-PCR)was based on the detection of a fluorescent reporter molecule that increases as PCR product accumulates with each cycle of amplification. It can be used to analysis the quantity of information of nucleic acid, and avoiding the pollution in traditional PCR. On the other hand, it has such excellences as high sensibility, specificity and accuracy. Recently, this technique was introduced and applied in food microbiology. The development of real-time FQ-PCR, its principle and advantages and disadvantages were described in detail,furthermore the application and prospects of real-time FQ-PCR in this field were reviewed. Future trends of real-time FQ-PCR techniques in food microbiology were also discussed in this paper.
Key words: Real-time fluorescent quantitative PCR； food microbe； quantitative detection；
文献注录：李勤,盛占武,孙志高*. 实时荧光定量PCR技术在食品微生物检测和研究中的应用 [J]. 四川食品与发酵. 2006, 05: 9-12.
报/刊名：《四川食品与发酵》，发表于2006 / 第 05 期。
文献类型： [J]
页码：第 9-12 页 / 共4 页