作者:向素琼,龚桂枝,郭启高,汪卫星,李春艳,李晓林,梁国鲁
作者单位:中国农业科学院柑桔研究所,西南大学园艺园林学院
摘要:果树2n配子育种较其他植物起步晚,尤其是利用2n花粉进行育种的研究在柑橘上还无先例。试验中初步观察了柑橘属部分品种的2n花粉自然发生情况,证实了柑橘属内未减数花粉的存在。同时通过秋水仙碱不同浓度与作用时间处理促进了柑橘2n花粉的发生,将长寿沙田柚2n花粉发生比例由当年的自然发生率0.87%提高到9.76%。初步观察了2n花粉离体萌发状况,其萌发率与花粉管伸长速度较为正常,但萌发启动比单倍性花粉慢。
关键词:柑橘,多倍体,2n花粉
Title: SPONTANEOUS GENERATION OF 2n POLLEN INCITRUSAND INDUCTION OF 2n POLLEN INCITRUS GRANDIS
Authors: XIANG Su-qiong,GONG Gui-zhi,GUO Qi-gao,WANG Wei-xing,LI Chun-yan,LI Xiao-lin,L IANG Guo-lu
Abstract: Observation of spontaneous occurrence of 2n pollen in a fewC itrusspec ies confirm ed the existence of unreduced pollen in them.Treatm ent ofC itrus grandisw ith d ifferent concentrations of colch ic ine and at d ifferent tim e for pollen chromosom e doub ling induction en-hanced the 2n pollen frequency from 0.87%(spontaneous occurrence) to 9.76%.Exam ination ofin vitrogerm ination of these 2n pollen grains revealed that the ir germ ination percentage and pollen tube elongation rate were w ith in the norm al range,though the start of germ ina-tion was delayed som ewhat as compared w ith the hap loid gam etes.
Key words: C itrus; polyp loid; 2n pollen;
文献注录:向素琼,龚桂枝,郭启高,汪卫星,李春艳,李晓林,梁国鲁. 柑橘属2n花粉自然发生与沙田柚2n花粉诱导研究 [J]. 西南农业大学学报(自然科学版). 2005, 05: 51-55.
基金: 重庆市自然基金资助项目(8573)
报/刊名:《西南农业大学学报(自然科学版)》,发表于2005 / 第 05 期。
文献类型: [J] (文献级别:核心刊物)
页码:第 51-55 页 / 共5 页