作者：彭军,周常勇,唐科志,谭万忠
作者单位：中国农业科学院柑桔研究所,西南农业大学植物保护学院
摘要：柑橘裂皮病是由柑橘裂皮病类病毒(Citrusexocortisviroid,CEVd)引起的一种重要的柑橘病害。分别采用快速微量核酸提取法和SDS-KAc抽提法在表现症状的Etrog香橼中提取核酸,采用一步RT-PCR法对CEVd进行检测,结果显示SDS-KAc法可以消除带毒样品中非特异性条带。从嫁接接毒的铜水72-1锦橙植株的接穗部取嫩叶、老叶、皮,从砧木部茎杆上取老皮以及根皮分别提取总核酸进行RT-PCR检测,分析CEVd在甜橙体内的分布情况。初步检测结果表明在接穗的嫩叶、老叶、皮,砧木部茎杆的老皮和根皮上都可以检测到CEVd,以接穗部叶和皮检出稳定性高。
关键词：柑橘裂皮病类病毒,分布,甜橙,RT-PCR检测
Title: Preliminary study on One-step RT-PCR detection of Citrus exocortis viroid (CEVd) and its distribution in different parts of sweet orange
Authors: PENG Jun, ZHOU Chang-yong, TANG Ke-zhi, TAN Wan-zhong
Abstract: Citrus exocortis is an important viroid disease. Preliminary study on comparison of Fast Micro-extraction method and SDS-KAc method,detecting CEVd in Etrog citron with symptoms by One-step RT-PCR, revealed that SDS-KAc method was preferable for eliminating non-specific band. Distribution of CEVd in the young leaf, mature leaf, bark and rootlet was monitored by One-step RT-PCR. The results indicated that CEVd could be detected in all parts monitored by One-step PT-PCR. Considering stability, leaf and bark of scion are more suitable to be used for detection of CEVd by One-step RT-PCR.
Key words: Citrus exocortis viroid； Distribution； Sweet orange； RT-PCR detection；
文献注录：彭军,周常勇,唐科志,谭万忠. 柑橘裂皮病类病毒的一步RT-PCR法检测及其在甜橙体内的分布 [J]. 果树学报. 2005, 06: 148-151.
报/刊名：《果树学报》，发表于2005 / 第 06 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 148-151 页 / 共4 页