作者：叶庆亮,彭爱红,曹立,江东,钟广炎
作者单位：西南大学园林园艺学院,中国农业科学院柑桔研究所
摘要：分别以柚类、柠檬、甜橙、枳壳和宽皮橘的果皮、果肉、枝皮、老叶、嫩叶、树根、子房、花瓣,尤其是以果胶含量丰富的岩溪晚芦果皮等作试材,将两次LiCl沉淀法加以改良,加入适量CaCl2,找到一种柑橘类果树总RNA优异且通用的提取方法,所得RNA的质量和产量均较高。经差减文库构建、连接、电转化,成功获得了目标片段。
关键词：柑橘,RNA提取,CaCl2处理,果胶,减法文库
Title: An Effective and General Protocol for the Isolation of Total RNA From the Tissues of Citrus Trees
Authors: YE Qing-hong,PENG Ai-hong,CAO Li,JIANG Dong,ZHONG Guang-yan
Abstract: Many samples,such as pulp,epicarp,tender leaves,old leaves,ovary,petal et al were tested respectively,especially the epicarp of Yanxiwanlu fruit very rich in polysacchrides.An effective and general protocol for the isolation of total RNA from the tissues of citrus trees was worked out.The protocol,based on two lithium chloride(LiCl) precipitations,is approved by some steps and added with appropriate CaCl2 during RNA extraction.The results Indicated that the RNA is of high quality,as confirmed by isolation of mRNA and subtraction construction of cDNA libraries.
Key words: Citrus； RNA extraction； CaCl2 treatment； pectate； subtraction library；
文献注录：叶庆亮,彭爱红,曹立,江东,钟广炎. 柑橘类果树组织总RNA优异且通用的提取方法 [J]. 江西农业大学学报. 2007, 05: 732-735+739.
基金: 科技部“973”重大基础研究前期研究专项(2005CCA02900);市自然科学基金(SCTC2005BA1010)
报/刊名：《江西农业大学学报》，发表于2007 / 第 05 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 732-735+739 页 / 共5 页