作者：徐小峰,周常勇,宋震,王雪峰,周彦
作者单位：西南大学植物保护学院,中国农业科学院柑桔研究所
摘要：对4种柑橘衰退病毒源及其31个单蚜传毒分离株的CP基因做了限制性片段长度多态性(RFLP)和单链构象多态性(SSCP)分析,并对其中8个单蚜传毒分离株的CP基因进行了序列分析。实验明确了所研究柑橘衰退病毒(Citrus triste-za virus,CTV)的CPG/Hinf I RFLP组群和CPG/SSCP模式,二者能较好地对应并有效验证了单蚜传毒对CTV毒源的分离纯化作用;CP基因序列分析结果表明,相同CPG/Hinf I RFLP组群的单蚜传毒分离株间具有高度的序列相似性,而不同CPG/Hinf I RFLP组群单蚜传毒分离株间则存在较大差异;通过与已知生物学特性CTV分离株比较,初步建立了上述CP基因分子特征与病毒生物学特性之间的联系。
关键词：柑橘衰退病毒,单蚜传毒,CP基因,限制性片段长度多态性,单链构象多态性
Title: Molecular characteristics of CP genes of 4 isolates of Citrus tristeza virus and their sub-isolates after single aphid transmission
Authors: XU Xiao-feng,ZHOU Chang-yong,SONG Zhen,WANG Xue-feng,ZHOU Yan
Abstract: Restriction fragment length polymorphism(RFLP) and single strain conformation polymorphism(SSCP) assays on coat protein(CP) genes of 4 CTV-isolates and their 31 sub-isolates after single aphid transmission were conducted,and CP genes of the 8 sub-isolates were sequenced.CPG/Hinf I RFLP groups and SSCP patterns of all the CTV were discovered,and both demonstrated that single aphid transmission was an effect way to separate CTV isolates.The results of sequencing showed that CP genes of sub-isolates with the characteristic of the same RFLP group had high homomorphism,while these fallen into different groups had lower homomorphism.The relationship among these molecular characteristics of CP genes and biological cha-racters of virus were preliminarily suggested by comparison with identified CTV isolates based upon typical symptoms.
Key words: Citrus tristeza virus； single aphid transmission； CP gene； RFLP； SSCP；
文献注录：徐小峰,周常勇,宋震,王雪峰,周彦. 4种柑橘衰退病毒源单蚜传毒分离株CP基因的分子特征 [J]. 植物病理学报. 2007, 01: 25-30.
基金: 国家自然科学基金资助项目(30471205)
报/刊名：《植物病理学报》，发表于2007 / 第 01 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 25-30 页 / 共6 页