作者:青玲,杨水英,孙现超,王进军,周常勇*
作者单位:西南大学植物保护学院,重庆市植物病害生物学重点实验室,中国农业科学院柑桔研究所
摘要:采用抗原直接包被酶联免疫吸附测定法(ELISA)对采自重庆近郊的34个葎草样品进行了主要病毒种类的检测。其中马铃薯Y病毒(Potato virus Y,PVY)的侵染最普遍,其阳性检出率达44.12%;马铃薯X病毒(Potatovirus X,PVX)的阳性检出率最低,仅为26.47%,其余5种病毒,烟草花叶病毒(Tobacco mosaic virus,TMV)、黄瓜花叶病毒(Cucumber mosaic virus,CMV)、番茄花叶病毒(Tomato mosaic virus,ToMV)、芜菁花叶病毒(Turnipmosaic virus,TuMV)及蚕豆萎蔫病毒2号(Broad bean wilt virus2,BBWV-2)的阳性检出率均为35.29%。葎草样品受多种病毒的复合侵染现象非常严重,15个阳性样品中病毒复合侵染率为80%,其中75%的样品检测到7种病毒复合侵染。
关键词:葎草,病毒,复合侵染,检测
Title: Detection of several co-infected viruses in Humulus scandens
Authors: QING Ling,YANG Shui-ying,SUN Xian-chao,WANG Jin-jun,ZHOU Chang-yong
Abstract: Thirty four samples of Humulus scandens with viral symptoms collected from the outskirts of Chongqing were tested for main virus types by using antigen direct coated ELISA.Seven kinds of viruses,including Tobacco mosaic virus(TMV),Cucumber mosaic virus(CMV),Tomato mosaic virus(ToMV),Turnip mosaic virus(TuMV),Broad bean wilt virus 2(BBWV-2),Potato virus X(PVX) and Potato virus Y(PVY),were detected in 15 samples.The infection rate of PVX was the lowest(26.47%),while the infection rate of PVY was t...
Key words: Humulus scandens; virus; co-infection; detection;
文献注录:青玲,杨水英,孙现超,王进军,周常勇*. 从葎草中检出复合侵染的多种病毒 [J]. 植物保护. 2009, 04: 138-140.
基金: 科技部科技基础性工作专项(2006FY111000);西南大学博士后基金(207001)资助项目
报/刊名:《植物保护》,发表于2009 / 第 04 期。
文献类型: [J]
页码:第 138-140 页 / 共3 页