作者：孙现超,赵刚,周常勇,青玲
作者单位：西南大学植物保护学院植物病毒学研究室,国家柑橘工程技术研究中心,中国农业科学院柑桔研究所
摘要：利用RT-PCR技术从感染温州蜜柑萎缩病毒的叶片中扩增出SDV大外壳蛋白(Large coat protein,CPL),将CPL克隆到pGEM-T载体后进行测序分析。DNA序列分析表明,SDV FJ的CPL基因cDNA序列全长为1329bp,编码443个氨基酸。与同属其他病毒序列对比分析结果显示,所得序列与日本报道的SDV S-58株系核苷酸和氨基酸同源性最高,分别为98.1%和98.6%。核苷酸序列与S-58相比25处发生了替换突变,其中T和C之间的替换频率最高,占绝对优势。
关键词：温州蜜柑萎缩病毒,大外壳蛋白,克隆,分析
Title: Cloning and sequencing of the large coat protein gene of Satsuma dwarf virus
Authors: SUN Xian-chao,ZHAO Gang, ZHOU Chang-yong, QING Ling
Abstract: The large coat protein (CPL) gene of Satsuma dwarf virus Fengjie isolate (SDV FJ) was amplified by RT-PCR and cloned into vector pGEM-T. Sequence analysis showed that the cDNA of SDV-FJ CPL was 1 329 bp, encoding a protein of 443 amino acids. Comparison of the sequence with those from other viruses of genus Sadwavirus showed high identities (98.1% and 98.6%) of nucleotides and amino acids between SDV FJ and SDV S-58 reported in Japan. Totally, 25 nucleotide differences were found between the CPL of SDV FJ and SDV S-58, among which the mutations between T and C account for a high percentage.
Key words: Satsuma dwarf virus； Large coat protein； Clone； Analysis
文献注录：孙现超,赵刚,周常勇,青玲. 温州蜜柑萎缩病毒大外壳蛋白基因克隆分析 [J]. 果树学报. 2010, 03: 457-460.
基金: 重庆市自然科学基金(CSTC.2007BB1349);西南大学博士启动基金(SWUB2006042)
报/刊名：《果树学报》，发表于2010 / 第 03 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 457-460 页 / 共4 页