作者：杨方云,魏朝富,刘英
作者单位：中国农业科学院柑桔研究所,西南大学资源环境学院
摘要：对2年生枳[Poncirus trifoliate(L.)Raf.]砧无病毒奉节72-1脐橙[Citrus sinensis(L.)Osbeck cv.Fengjie 72-1 navel]嫁接苗进行持续性干旱胁迫处理,研究干旱胁迫期间甜橙叶片保护酶活性和丙二醛(MDA)含量随土壤和植株水分变化的生理适应性。结果表明,干旱胁迫明显导致了过氧化氢酶(CAT)活性下降,过氧化物酶(POD)和超氧化物歧化酶(SOD)活性上升,而MDA含量变化不明显。干旱胁迫初期或轻度胁迫期,保护酶活性已具有明显的适应性反应;当处于中度胁迫时,POD和SOD依然保持很高活性。在防止MDA产生中,CAT的作用不明显,POD和SOD起主要的协同作用。
关键词：干旱胁迫,甜橙,保护酶
Title: Protective enzyme systems in orange leaves under drought stress
Authors: YANG Fang-yun,WEI Chao-fu,LIU Ying
Abstract: Two years old of Citrus sinensis(L.) Osbeck cv.Fengjie 72-1 navel oranges grafted onto Poncirus trifoliate(L.) Raf.were planted in containers filled with artificial nutrient soil(sand ∶ peat ∶ rice husk=5∶4∶1).Different duration of continuous drought stress,0,2,4,6,8,10,12,or 14 day,was imposed on different treatments.For each drought treatment,soil sample and five plants were picked out periodically to determine the soil water content and water potential(Ψ_l),relative water content(RWC),activities of catalase(CAT),peroxidase(POD) and superoxide dismutase(SOD),and content of malondialdehyde(MDA) of the leaves.Meantime,physiological responses of protective(enzyme) activities and content of MDA to soil and plant water changes were studied.Results showed that activity of CAT was depressed,while activity of POD and SOD was promoted and content of MDA changed little under drought stress.At the earlier stage of drought stress or under light drought stress,the activities of protective enzymes are of evident adaptive(response).Being in a condition of moderate stress,POD and SOD retained high activity.POD and SOD together played a dominant synergetic effect in restraining the produce of MDA,while CAT contributed little.
Key words: drought stress； orange； protective enzyme；
文献注录：杨方云,魏朝富,刘英. 干旱胁迫下甜橙叶片保护酶体系的变化研究 [J]. 植物营养与肥料学报. 2006, 01: 119-124.
报/刊名：《植物营养与肥料学报》，发表于2006 / 第 01 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 119-124 页 / 共6 页