作者:姚廷山,周彦,李中安,唐科志,杨方云,肖田,胡军华,周常勇*
作者单位:中国农业科学院柑桔研究所,国家柑桔工程技术研究中心
摘要:从四川、湖北两省收集的柑桔病样分离出2个溃疡病菌株,经生物学和PCR鉴定后,分别针刺接种于纽荷尔脐橙、福本脐橙、卡拉卡拉脐橙和春见桔橙等4个柑桔品种;接种2 d后调查发病情况,使用DPS软件8.01的Duncan新复极差法进行分析。结果表明,接种后4 d是最佳观察时期,4个柑桔品种分别接种上述菌株后发病率无明显差异,老叶和成叶的发病率要高于嫩叶的发病率,叶片不同部位之间的发病率也基本相当,桔橙的抗病性要高于脐橙的抗病性。
关键词:柑桔溃疡病菌,鉴定,离体叶,接种,致病性
Title: Preliminary Study on Pathogenicity of Xanthomonas axonopodis pv. Citri
Authors: YAO Ting-shan,ZHOU Yan,LI Zhong-an,TANG Ke-zhi,YANG Fang-yun,XIAO Tian,HU Jun-hua,ZHOU Chang-yong
Abstract: Two citrus canker strains were isolated from the samples collected from Sichuan and Hubei provinces.After identification by PCR,the two strains were vaccinated in 4 citrus varieties such as newhall navel orange,fukumoto navel orange,cara.cara navel orange and kivomi tangor by acupuncture.Two days’ later,the incidence was investigated.After analysis by DPS software 8.01 Duncan LSR method,the results showed that 4 days after inoculation was the best period for observation.There were no significant difference among the 4 citrus varieties after inoculation,no difference were found out in a same leaf,either.Disease ratio in old leaves and young leaves were higher than that in tender leaves.Disease resistant of hybrid of mandarin orange was superior to the resistance of navel orange.
Key words: Citrus bacterial canker strain; Identification; Excised leaf; Inoculation; Pathogenicity;
文献注录:姚廷山,周彦,李中安,唐科志,杨方云,肖田,胡军华,周常勇*. 柑桔溃疡病菌致病性研究初探 [J]. 西南农业学报. 2009, 06: 1616-1618.
基金: 国家科技支撑计划项目(2007BAD47B03);市重大科技专项(CSTC,2007AA1024);2009年农作物病虫鼠害疫情监测与项目资助
报/刊名:《西南农业学报》,发表于2009 / 第 06 期。
文献类型: [J] (文献级别:核心刊物)
页码:第 1616-1618 页 / 共3 页