作者：
摘要：This research proposes a batch in vitro rumen apparatus modified from Daisy incubator and procedures of measuring feeddigestion by using nylon bag to replace the traditional centrifuging and filtration methods, for the purpose of studyingruminal fermentation. The apparatus consists of 8 sets of airtight reaction vessels which rotated at a speed of 1 r/min andlocated inside a thermostatic cabin. Optimum procedures was proved to be that the volume of incubation fluid was400 mL; fineness and size of test feed were 0.125-0.25 mm and 0.4 g, respectively; amount of bags in one vessel was 6;fermentation diet in a single incubation was 4-5 g. Comparing experiments showed that fermentation speed attained withthe apparatus was about 15-20% greater than that attained with flask-shaking machine method (revolutionary speed is100 r/min) and more accurate results could be obtained with the new apparatus. In conclusion, this apparatus couldprovide an efficient mixing action, and the procedure could allow sensitive detection of differences in ruminal fermentationin a time-saving way.
关键词：in vitro apparatus;ruminal fermentation;nylon bag;
文献注录：. A Proposed Batch In vitro Apparatus for Conducting Rumen Fermentation Experiment [J]. Agricultural Sciences in China. 2008, 07: 887-894.
报/刊名：《Agricultural Sciences in China》，发表于2008 / 第 07 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 887-894 页 / 共8 页