作者：Lim, Wallace B. L.
摘要：<正> The very virulent infectious bursal disease virus (vvIBDV) strain Gx was isolated from a poutl-try farm in Guangxi Province, China, during 1996. The mortality in the infected flock was 80% and occurred 5 days after immunization with serotype I IBD vaccine. The results of antigen-capture ELISA (AC-ELISA), pathogenicity testing, cloning and sequence analysis of the VP2 gene showed that the deduced amino acid sequence of strain Gx VP2 was the same as vvIBDV UK661, which is considered as a reference strain for European vvIBDVs. The antigenicity of the Gx strain was the same as an European vvIBDV strain 849. The EID50 of Gx virus was 10-8.25/0. 2 ml, and the mortality was 64% when 4 week-old SPF chickens were challenged at dosage of 2×10~3EID50. We have demonstrated that the IBDV strain Gx isolated in China is vvIBDV according to European standards.
关键词：Infectious bursal disease;Very virulent strain(Gx);Pathogenicity;VP2 gene;
文献注录：Lim, Wallace B. L.. Pathogenic Antigenic and Molecular Characterization of the Very Virulent Strain(Gx) of Infectious Bu [J]. Agricultural Sciences in China. 2003, 05: 97-103.
报/刊名：《Agricultural Sciences in China》，发表于2003 / 第 05 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 97-103 页 / 共7 页