作者:
摘要:<正> Porcine colostrum and milk were separated into the acid-soluble fraction(SF)and casein fraction(CF)by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components were determined by incubating bovine trypsin or chymotrypsin in a medium. The inhibition of insulin-like growth factor I(IGF-I)and epidermal growth factor(EGF)degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid(TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The SF was higher in inhibitory potency than CF. The present study revealed that the protease inhibitors in porcine colostrum, milk-derived and colostrum-specific, existed mainly in SF.
关键词:Porcine colostrum;Protease inhibitors;Inhibitory capacity;Growth factors;
文献注录:. A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum [J]. Agricultural Sciences in China. 2003, 07: 106-111.
报/刊名:《Agricultural Sciences in China》,发表于2003 / 第 07 期。
文献类型: [J] (文献级别:核心刊物)
页码:第 106-111 页 / 共6 页