标题:A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum

作者:

摘要:<正&gt; Porcine colostrum and milk were separated into the acid-soluble fraction(SF)and casein fraction(CF)by centrifuge. Trypsin and chymotrypsin inhibitory capacity in porcine colostrum, milk and their components were determined by incubating bovine trypsin or chymotrypsin in a medium. The inhibition of insulin-like growth factor I(IGF-I)and epidermal growth factor(EGF)degradation in pig small intestinal contents by porcine colostrum was measured by incubating iodinated IGF-I or EGF. Degradation of labeled IGF-I or EGF was determined by monitoring the generation of radioactivity soluble in 30% trichloroacetic acid(TCA). The results showed that porcine colostrum had high levels of trypsin and chymotrypsin inhibitory activity and increased the stability of IGF-I and EGF in pig intestinal contents. The SF was higher in inhibitory potency than CF. The present study revealed that the protease inhibitors in porcine colostrum, milk-derived and colostrum-specific, existed mainly in SF.

关键词:Porcine colostrum;Protease inhibitors;Inhibitory capacity;Growth factors;

文献注录:. A Study on the Inhibitory Potency of Protease Inhibitorsin Porcine Colostrum [J]. Agricultural Sciences in China. 2003, 07: 106-111.

报/刊名:Agricultural Sciences in China》,发表于2003 / 第 07 期。

文献类型: [J] (文献级别:核心刊物

页码: 106-111 页 / 共6

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