作者:
摘要:<正> A novel proventriculopathogic variant (isolate ZJ971) of infectious bronchitis virus (IBV) was identified from enlarged proeventriculus of the sick chickens in the study. The S gene cDNA segment with 3.6 kb in length was amplified by RT-PCR with special primers from the ZJ971 viral isolate of (IBV) and cloned into plasmid pBluescript SK( + ). The recombinants containing S gene of IBV-ZJ971 isolate were identified by digestion of restriction enzyme EcoRI, BamHI and PCR amplification. The cloned S gene from isolate IBV-ZJ971 was composed of 3492 bp in length encoding for a polypeptide of 1080 amino acids. Comparing the
关键词:Avian;Proventriculopathogic infectious bronchitis virus;S gene;Cloning;
文献注录:. Cloning and Sequencing of S Gene of Novel Variant of Infectious Bronchitis Virus ZJ971 Isolates in C [J]. Agricultural Sciences in China. 2002, 01: 105-111.
报/刊名:《Agricultural Sciences in China》,发表于2002 / 第 01 期。
文献类型: [J] (文献级别:核心刊物)
页码:第 105-111 页 / 共7 页