作者：
摘要：<正> Conditions for electrical parthenogenetic activation of porcine oocytes matured in vitro and in vitro culture systems of porcine embryo were studied. The best results were achieved under the conditions of electrical field strength and the pulse duration at 130Vmm-1/80 us, with a blastocyst development rate of (20.12 ± 8.18) % (P > 0.05). No significant difference was found between treatments of multiple pulses and a single pulse (P > 0.05). Parthenogenetic embryos were cultured with different methods and air conditions for 7 days in vitro, blastocyst development rate of embryos with changed culture media [ (26.44 ± 8.35) % ] or changed media with 10% fetal bovine serum (FBS) [ (17.68 ± 5.39)% ] on the fifth day showing no significant difference from that of embryos without change of culture media [ (25.30 ± 7.55) % , P > 0.05 ], while cell numbers of blastocysts from embryos with changed culture media (15.78 + 5.46 and 14.55 ± 4.81) were significantly lower than number of blastocysts from embryos w
关键词：Porcine;Oocytes;Culture in vitro;Electrical activation;Embryo culture;
文献注录：. Studies on Electrical Activation of Porcine Oocytes Matured in vitro and Embryo Culture Systems [J]. Agricultural Sciences in China. 2002, 10: 105-110.
报/刊名：《Agricultural Sciences in China》，发表于2002 / 第 10 期。
文献类型： [J] （文献级别：核心刊物）
页码：第 105-110 页 / 共6 页