作者:Bassan MM, Mourao FDA, Miyata LY, Mendes BMJ.
作者单位:USP
Abstract: The objective of this work was to optimize in vitro plant regeneration via organogenesis from tissues of adult \'Hamlin\', \'Pera\', and \'Valencia\' sweet orange plants. Explants were grown in EME culture medium with different concentrations of 6-benzylaminopurine (BAP) and naphthaleneacetic acid (NAA), at 27 degrees C in the absence of light for 50 days, followed by a 16-hour photoperiod for 20 days. Regeneration was assessed 50 and 70 days after in vitro culture. Organogenesis in cultivars Hamlin and Valencia was promoted by EME supplemented with BAP, while NAA showed no apparent effect.
Key words: Citrus sinensis; adventitious buds; biotechnology; plant growth regulators
文献注录:Bassan MM, Mourao FDA, Miyata LY, Mendes BMJ.. In vitro organogenesis from internodal segments of adult sweet orange plants [J]. Pesquisa Agropecuaria Brasileira. 2011, 06: 627-674.
报/刊名:《Pesquisa Agropecuaria Brasileira》,发表于2011 / 第 06 期。
文献类型: [J]
页码:第 627-674 页 / 共48 页