作者：Febres VJ, Lee RF, Moore GA
摘要：Grapefruit (Citrus paradisi) transgenic plants transformed with a variety of constructs derived from the Citrus tristeza virus (CTV) genome were tested for their resistance to the virus. Most transgenic lines were susceptible (27 lines), a few were partially resistant (6 lines) and only one line, transformed with the 30 end of CTV was resistant. Transgene expression levels and siRNA accumulation were determined to identify whether the resistance observed was RNA-mediated. The responses were varied. At least one resistant plant from a partially resistant line showed no steady-state transgene mRNA, siRNA accumulation and no viral RNA, implicating posttranscriptional gene silencing (PTGS) as the mechanism of resistance. The most resistant line showed no transgene mRNA accumulation and promoter methylation of cytosines in all contexts, the hallmark of RNA-directed DNA methylation and transcriptional gene silencing (TGS). The variety of responses, even among clonally propagated plants, is unexplained but is not unique to citrus. The genetics of CTV, host response or other factors may be responsible for this variability.
关键词：Citrus tristeza virus , DNA methylation , Plant resistance , RNA interference
文献注录：Febres VJ, Lee RF, Moore GA. Transgenic resistance to Citrus tristeza virus in grapefruit [J]. Plant Cell Reports. 2008, 01: 93-104.
报/刊名：《Plant Cell Reports》，发表于2008 / 第 01 期。
文献类型： [J]
页码：第 93-104 页 / 共12 页