作者：López C, Cervera M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L.
摘要：Mexican lime plants transformed with the 3′-terminal 549 nucleotides of the Citrus tristeza virus (CTV) genome in sense, antisense and intron-hairpin formats were analysed for transgenederived transcript and short interfering RNA (siRNA) accumulation, and for CTV resistance. Propagations from all sense, antisense and empty-vector transgenic lines were susceptible to CTV, except for a single sense-line plant with a complex transgene integration pattern that showed transgene-derived siRNAs in association with low levels of the transgene-derived transcript. In contrast, nine of 30 intron-hairpin lines showed CTV resistance, with 9%–56% of bud-propagated plants, depending on the line, remaining uninfected on graft inoculation, and the others being susceptible. Although resistance was always associated with the presence of transgene-derived siRNAs, their level in different sense and intron-hairpin transformants was variable irrespective of the response to CTV infection. In intron-hairpin lines with single transgene integration, CTV resistance was correlated with low accumulation of the transgene-derived transcript rather than with high accumulation of transgene-derived siRNAs.
文献注录：López C, Cervera M, Fagoaga C, Moreno P, Navarro L, Flores R, Peña L.. Accumulation of transgene-derived siRNAs is not sufficient for RNAi-mediated protection against Citrus tristeza virus in transgenic Mexican lime [J]. Molecular Plant Pathology. 2010, 01: 33-41.
报/刊名：《Molecular Plant Pathology》，发表于2010 / 第 01 期。
文献类型： [J]
页码：第 33-41 页 / 共9 页