作者：Leandro Peña, Magdalena Cervera, José Juárez, Antonio Navarro, José A. Pina, Nuria Durán-Vila, Luis Navarro.
摘要：Transgenic sweet orange (Citrus sinensis L. Osbeck) plants have been obtained by Agrobacterium tumefaciens-mediated gene transfer. An hypervirulent A. tumefaciens strain harboring a binary vector that contains the chimeric neomycin phosphotransferase H (NPT II) and β-glucuronidase (GUS) genes was cocultivated with stem segments from in vivo grown seedlings. Shoots regenerated under kanamycin selection were harvested from the stem segments within 12 weeks. Shoot basal portions were assayed for GUS activity and the remaining portions were shoot tip grafted in vitro for production of plants. Integration of the GUS gene was confirmed by Southern analysis. This transformation procedure showed the highest transgenic plant production efficiency reported for Citrus.
关键词：Citrus sinensis L. Osbeck cv. Pineapple ; Sweet orange ; Agrobacterium tumefacie
文献注录：Leandro Peña, Magdalena Cervera, José Juárez, Antonio Navarro, José A. Pina, Nuria Durán-Vila, Luis Navarro.. Agrobacterium-mediated transformation of sweet orange and regeneration of transgenic plants [J]. Plant Cell Reports. 1995, 10: 616-619.
报/刊名：《Plant Cell Reports》，发表于1995 / 第 10 期。
文献类型： [J]
页码：第 616-619 页 / 共4 页