作者：Luis Gustavo de Paoli, Raquel Luciana Boscariol Camargo, Ricardo Harakava, Beatriz Madalena Januzzi
摘要：The objective of this work was to produce 'Valencia' sweet orange transgenic plants with the cecropin MB39 gene controlled by a phenylalanine ammonia-lyase gene promoter from citrus in order to direct gene expression in xylem vessels. The genetic transformation was mediated by Agrobacterium tumefaciens with the co-culture of epicotyl segments. Eleven transgenic plants were selected by PCR with the amplification of a 189 bp fragment, which were acclimatized to greenhouse. The integration of the transgene was confirmed in three plants by Southern blot analysis.
关键词：De Paoli LG, Camargo RLB, Harakava R, Mendes BMJ, Mourao FDA.
文献注录：Luis Gustavo de Paoli, Raquel Luciana Boscariol Camargo, Ricardo Harakava, Beatriz Madalena Januzzi. Genetic transformation of \'Valencia\' sweet orange with the cecropin MB39 gene [J]. Pesquisa Agropecuária Brasileira. 2007, 11: 1663-1666.
报/刊名：《Pesquisa Agropecuária Brasileira》，发表于2007 / 第 11 期。
文献类型： [J]
页码：第 1663-1666 页 / 共4 页