标题:Bud emergence and shoot growth from mature citrus nodal stem segments

作者:Marutani-Hert M, Evens TJ, McCollum GT, Niedz RP.

摘要:Bud emergence and shoot growth from adult phase citrus nodal cultures were studied using Citrus mitis (calamondin), Citrus paradisi (grapefruit), and Citrus sinensis (sweet orange). The effects of 6-benzyladenine (BA), indole 3-acetic acid (IAA), and citrus type on shoot quality and growth of mature bud explants from greenhouse grown trees were determined using a 2-component mixtureamount 9 citrus type experiment. BA increased shoot number and IAA improved shoot growth. The best shoot quality (fewer shoots but large shoots) was obtained with 1μM IAA for calamondin, 15.5 lM IAA for sweet orange, and 30μM IAA for grapefruit. Grapefruit exhibited substantial leaf abscission compared to calamondin and sweet orange. Four factors (AgNO3, silver thiosulphate (STS), CaNO3, or gelling) were screened individually for their efficacy in reducing leaf abscission. Five factors (AgNO3, gelling, MS ion concentration, plant growth regulator and venting) were investigated to identify potential combinations for reducing leaf abscission and maximizing shoot growth and bud emergence. The factor combination identified as most effective in minimizing leaf drop, promoting shoot growth, and maximizing bud emergence for grapefruit was 2 mg l-1 AgNO3, Gelrite, 1×MS ion concentration, 30μM IAA, and vented.

关键词:Citrus mitis ; Citrus paradisi ; Citrus sinensis ; Adult phase ; Nodal stem segm

文献注录:Marutani-Hert M, Evens TJ, McCollum GT, Niedz RP.. Bud emergence and shoot growth from mature citrus nodal stem segments [J]. Plant Cell, Tissue and Organ Culture (PCTOC). 2010, 01: 81-91.

报/刊名:Plant Cell, Tissue and Organ Culture (PCTOC)》,发表于2010 / 第 01 期。

文献类型: [J]

页码: 81-91 页 / 共11

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