作者：Mendes AFD, Cidade L, de Oliveira MLP, Otoni WC, Soares WD, Costa M.
摘要：Identification of beta-lactam antibiotics that have negligible effects on plant regeneration is a critical step towards the establishment of a reliable Agrobacterium-mediated transformation protocol for perennial trees. In the present report, we have evaluated the effects of the novel beta-lactam antibiotics meropenem and timentin on plant regeneration of a perennial woody fruit plant, Citrus sinensis, in comparison with the commonly used betalactam cefotaxime. It was observed that, in contrast to cefotaxime, meropenem and timentin had a positive or no detrimental effect on the shoot regeneration from epicotyl explants. Residual effects of the beta-lactams from shoot regeneration medium also affected the subsequent ability of the roots to elongate. The addition of meropenem and/or timentin in the rooting medium mostly improved or did not affect the rooting ability of the adventitious shoots. These data indicated that meropenem and timentin can positively replace cefotaxime in Agrobacterium-mediated transformation of C. sinensis.
关键词：Agrobacterium tumefaciens ; Bactericidal activity ; Phytotoxicity ; Morphogenesi
文献注录：Mendes AFD, Cidade L, de Oliveira MLP, Otoni WC, Soares WD, Costa M.. Evaluation of novel beta-lactam antibiotics in comparison to cefotaxime on plant regeneration of Citrus sinensis L. Osb. [J]. Plant Cell, Tissue and Organ Culture (PCTOC). 2009, 03: 331-336.
报/刊名：《Plant Cell, Tissue and Organ Culture (PCTOC)》，发表于2009 / 第 03 期。
文献类型： [J]
页码：第 331-336 页 / 共6 页