作者：Duan YX, Guo WW, Meng HJ, Tao NG, Li DD, Deng XX.
摘要：Transformation and high efficient regeneration of transgenic plants from embryogenic calluses of Bingtang sweet orange [Citrus sinensis (L.) Osbeck] was reported. Embryogenic calluses were inoculated with Agrobacterium tumefaciens strain EHA105, harboring the binary Ti plasmid pROK II and carrying a neomycin phosphotransferase II (NPTII) gene, an intron β-glucuronidase (GUS) gene and the Arabidopsis APETALA1 (AP1) gene. Transformation treatment was with inoculation time of 30 min, co-culture of 3 d at 23 °C and supplementation of the co-culture medium with 2 mg dm-3 acetosyringone (AS). Kanamycin (50 mg dm-3) was effective to inhibit the growth of non-transformed calluses while it did not affect the transformed ones. The total number of transformed callus lines was 7 with 100 % embryo induction. High efficient regeneration of the transgenic embryos (88 % with 4 - 5 shoots per embryoid) was realized within 3 months. Integration of the transgene into the citrus genome was confirmed by histochemical GUS staining, polymerase chain reaction (PCR) analysis with AP1-specific primer and Southern blot hybridization with a 712 bp PCR fragment of AP1 as the probe.
关键词：Agrobacterium tumefaciens, Bingtang sweet orange, GUS, kanamycin, PCR, plantlet
文献注录：Duan YX, Guo WW, Meng HJ, Tao NG, Li DD, Deng XX.. High efficient transgenic plant regeneration from embryogenic calluses of Citrus sinensis [J]. Biologia Plantarum. 2007, 02: 212-216.
报/刊名：《Biologia Plantarum》，发表于2007 / 第 02 期。
文献类型： [J]
页码：第 212-216 页 / 共5 页