作者：敖小平, 胡新喜, 郭琛, 焦徕, 邓子牛, 熊兴耀.
摘要：Internodal stem segments from 30 d old seedlings of‘Dahong’sweet orange(Citrus sinensis Osbeck)were cultured on bud induction media，to study on the genetic transformation in Citrus with rolB gene．The best medium for induction was MS+3.0 mg/L 6-BA，which led to 95%of explants producing at least one bud，so this medium could be considered as the best for bud induction in plant regeneration．The co-culture mediumⅡ(MS+3 mg/L 6-BA+200μmol/L As)+selective mediumⅣ(MT+3 mg/L 6-BA+50 mg/L Kan+500 mg/L Cef)were the suitable combination for production of transgenic bud，and on which the ratio of buds induction could be 51%．Kan-resistant buds were micro grafted on in vitro grown seedlings of C.Sinensis×P.trifoliata，and 19 transgenic plants derived there well in the greenhouse.
关键词：‘Dahong’Sweet Orange；genetic transformation；rolB gene
Key words: 大红甜橙;遗传转化;rolB基因
文献注录：敖小平, 胡新喜, 郭琛, 焦徕, 邓子牛, 熊兴耀.. 用rolB基因转化大红甜橙的初步研究 [J]. 湖南农业大学学报(自然科学版). 2005, 06: 623-626.
报/刊名：《湖南农业大学学报(自然科学版)》，发表于2005 / 第 06 期。
文献类型： [J]
页码：第 623-626 页 / 共4 页