作者：曾黎辉, 徐海峰, 王会全, 吴少华, 朱艺萱.
摘要：The plant expression vectors pCAMBIA1301PMI and pBIPMI were constructed by substituting Escherichia coli phosphomannose-isomerase(PMI)gene for hpt gene of pCAMBIA1301 and gus gene of pBI121.Epicocyl explants of Xuegan sweet orange(Citrus sinensis L.Osbeck)were inoculated with Agrobacterium-tumefaciens EHA105-pCAMBIA1301PMI and EHA105-pBIPMI and subsequently selected on medium supplemented with a combination of 25 g/L mannose and 5 g/L sucrose as a carbon source.The transformation efficiency rate was 27.7%when transformed by pCAMBIA1301PMI and 12.7%by pBIPMI.Genetic transformation was confirmed by chlorophenol red assay and PCR,indicating that a new method for obtaining transgenic Xuegan plants was developed using PMI/mannose selection system.
关键词：phosphomannose-isomerase(PMI)gene; mannose; Xuegan sweet orange; genetic transfo
Key words: 6-磷酸甘露糖异构酶（PMI）基因;甘露糖;雪柑;转基因
文献注录：曾黎辉, 徐海峰, 王会全, 吴少华, 朱艺萱.. PMI基因作为选择标记的植物表达载体构建及其在雪柑转基因中的应用 [J]. 农业生物技术学报. 2008, 05: 858-864.
报/刊名：《农业生物技术学报》，发表于2008 / 第 05 期。
文献类型： [J]
页码：第 858-864 页 / 共7 页