作者：段艳欣, 李鼎立, 郭文武.
摘要：Threre are significant differences in tansformation effieiency between different citrus embryogenic cailuses. Embryogenic calluses of 11 citrus genotypes ( including Citrus reticulata, C. sinensis，C. Paradisi, Fortunella crassifolia, etc), were assessed for Agrobacterium tumefaciens mediated transformation potentials. GUS positive callus lines from 10 genotypes were produced and the transformation result indicated that there was remarkable relatvity between transformation efficiency and citrus callus browning. Dark brown callus also yielded higher transformation efficiency, while weak or no brown calluses gave lower one. Callus from C. reticulata brown severely, and the transformation efficiency reached up to 80%. Calluses from C. sinensis brown variously from borwn to weak or no brown, and the transformation efficiency was 0-20%. F. crossifolia and C. Paradise were between them, the transformation efficiency was 20% and 6%, respectively. Further study indicated that total phenolic content was positively correlated with citrus callus browning. As a result, total phenolic content affect citrus callus browning thus influence transformation efficiency. The possible mechenism was that total phenolic content was related to induction of vir genes and transfer of T一DNA.
关键词：citrus,Embryogenic callus,Browning,Transformation,Total phenolic content
Key words: 柑橘;胚性愈伤组织;褐化;转化;多酚
文献注录：段艳欣, 李鼎立, 郭文武.. 柑橘胚性愈伤组织的褐化与转化 [J]. 《2008园艺学进展（第八辑）——中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集》. 2008: 110-115.
报/刊名：《《2008园艺学进展（第八辑）——中国园艺学会第八届青年学术讨论会暨现代园艺论坛论文集》》，发表于2008
文献类型： [J]
页码：第 110-115 页 / 共6 页