作者：段艳欣, 范净, 郭文武.
摘要：‘Nanfeng’tangerine (Citrus reticulata), favorite for its sweet-fleshed and thin-rind fruit, is one of the oldest citrus cultivars in China. However, the decline of its fruit quality affected its commodity value. In order to explore a rapid and effective breeding programme for cultivar improvement, we performed Agrobacterium-mediated transformation of this cultivar using embryogenic callus induced from hypocotyl of seedling. Ti plasmid pROKII with the Arabidopsis APETALA1 (AP1) gene and a neomycin phosphotransferase II (NPTII) gene was used in this experiment. Totally, twenty-two resistant callus clusters were obtained with a mean number of 7.3 cell lines per plate. Further PCR and Southern blot analysis confirmed that transgenes were integrated into citrus genome. Though we have not succeeded in regenerating whole transgenic plants, this study indicate that embryogenic callus of‘Nanfeng’tangerine is ideal explants for genetic engineering.
关键词：Agrobacterium-mediated transformation, embryogenic callus,‘Nanfeng’tangerine, PC
Key words: 根癌农杆菌介导转化;胚性愈伤组织;南丰蜜橘;PCR分析;Southern;blotting检测
文献注录：段艳欣, 范净, 郭文武.. 南丰蜜橘胚性愈伤组织诱导及其转化研究 [J]. 中国农学通报. 2009, 08: 42-45.
报/刊名：《中国农学通报》，发表于2009 / 第 08 期。
文献类型： [J]
页码：第 42-45 页 / 共4 页