作者：段艳欣, 范净, 李鼎立, 郭文武.
摘要：To screen the transformants rapidly and efficiently and to obtain putative homozygous transgenie plants in a short time as soon as possible，the Agrobacterium tumefaciens mediated transformarion of embryogenic calli from Ponkan(Citrus reticulata Blanco)，Bingtang sweet orange(C sinensis Osbeck)and Murcott(c．reticulata×C sinensis)were conducted．The sensitivities of embryogenic calli and embryos to kanamycin were different．The growth of resistant tissues at different developmental stages was influenced by kanamycin．A scheme of five-step screening and regeneration of transformants was proposed．A large number of regenerated cell lines were obtained and checked by GUS assay．
关键词：Agrobacterium tumefaciens；GUS assay；kanamycin；citrus
Key words: 根癌农杆菌;GUs检测;卡那霉素;柑橘
文献注录：段艳欣, 范净, 李鼎立, 郭文武.. 柑橘转基因胚性愈伤组织的筛选与再生 [J]. 华中农业大学学报. 2008, 06: 768～771.
报/刊名：《华中农业大学学报》，发表于2008 / 第 06 期。
文献类型： [J]
页码：第 768～771 页 / 共1 页