作者：郭卫东, 周春丽, 路梅, 谢选松.
摘要：Tender and elder leaf-discs of fingered-citrus (Citrus medicaL. var.sarcodactylis(Noot) Swingle) were transformed withGusgene via biolistics method. The transient expression of Gus genewas used to analyse the transformation rate under different target distance. The results indicated that a higher transformation rate according to transient expression ofGusgene could be acquired from tender leaf-discs bombarded under 7.584 5×106Pa rupture pressure and 6 cm target distance. Transformation rate of elder-leaf was lower. Increasing bombardment times could not improve the transformation rate
关键词：Citrus medicaL. var.sarcodactylis; biolistics;Gusgene; transformation; transient
Key words: 佛手;基因枪;Gus基因;瞬时表达
文献注录：郭卫东, 周春丽, 路梅, 谢选松.. Gus基因在佛手叶片中的瞬间表达 [J]. 浙江师范大学学报(自然科学版). 2005, 04: 425-428.
报/刊名：《浙江师范大学学报(自然科学版)》，发表于2005 / 第 04 期。
文献类型： [J]
页码：第 425-428 页 / 共4 页