作者：Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.
作者单位：Univ Florida IFAS
摘要：'Mexican' lime (Citrus aurantifolia Swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolC promoter of Agrobacterium rhizogenes, Arabidopsis thaliana sucrose-H+ symporter (AtSUC2) gene promoter of Arabidopsis thaliana, rice tungro bacilliform virus (RTBV) promoter and sucrose synthase l (RSs1) gene promoter of Oryza sativa (rice). Histochemical beta-glucuronidase (GUS) analysis revealed vascular-specific expression of the GUS protein in citrus. The RTBV promoter was the most efficient promoter in this study while the RSs1 promoter could drive low levels of gus gene expression in citrus. These results were further validated by reverse transcription real-time polymerase chain reaction and northern blotting. Southern blot analysis confirmed stable transgene integration, which ranged from a single insertion to four copies per genome. The use of phloem-specific promoters in citrus will allow targeted transgene expression of antibacterial constructs designed to battle huanglongbing disease (HLB or citrus greening disease), associated with a phloem-limited Gram-negative bacterium.
关键词：Agrobacterium tumefaciens; AtSUC2; citrus; GUS; Mexican lime; rolC; RSs1; RTBV;
Authors: Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW
Abstract: \'Mexican\' lime (Citrus aurantifolia Swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolC promoter of Agrobacterium rhizogenes, Arabidopsis thaliana sucrose-H+ symporter (AtSUC2) gene promoter of Arabidopsis thaliana, rice tungro bacilliform virus (RTBV) promoter and sucrose synthase l (RSs1) gene promoter of Oryza sativa (rice). Histochemical beta-glucuronidase (GUS) analysis revealed vascular-specific expression of the GUS protein in citrus. The RTBV promoter was the most efficient promoter in this study while the RSs1 promoter could drive low levels of gus gene expression in citrus. These results were further validated by reverse transcription real-time polymerase chain reaction and northern blotting. Southern blot analysis confirmed stable transgene integration, which ranged from a single insertion to four copies per genome. The use of phloem-specific promoters in citrus will allow targeted transgene expression of antibacterial constructs designed to battle huanglongbing disease (HLB or citrus greening disease), associated with a phloem-limited Gram-negative bacterium.
Key words: Agrobacterium tumefaciens; AtSUC2; citrus; GUS; Mexican lime; rolC; RSs1; RTBV; transformation
文献注录：Dutt M, Ananthakrishnan G, Jaromin MK, Brlansky RH, Grosser JW.. Evaluation of four phloem-specific promoters in vegetative tissues of transgenic citrus plants [J]. Tree Physiology. 2012, 32(1): 83-93.
报/刊名：《Tree Physiology》，发表于2012 / 第 32(1) 期。
文献类型： [J]
页码：第 83-93 页 / 共11 页