作者：Carneiro CEA, Molinari HBC, Andrade GA, Pereira LFP, Vieira LGE.
作者单位：Instituto Agronômico do Paraná
摘要：The objective of this work was to evaluate the susceptibility to glufosinate ammonium of transgenic plants of Swingle citrumelo with high proline production. The mutant gene of the enzyme Delta(1)-pyrroline-5-carboxylate synthetase (P5CS), the rate-limiting enzyme in proline biosynthesis, was inserted into Swingle citrumelo plants. The expression of the gene p5cs caused up to 5-fold increase on the proline content in leaf tissues of transgenic plants treated with 200 mu M glufosinate ammonium, when compared with control plants. Leaves of transgenic plants accumulated higher amounts of NH4+ than the nontransgenic control. The herbicide toxicity was evaluated using leaf disks cultivated in MS medium, containing different concentrations of glufosinate ammonium. The severity of the chlorosis, observed in leaf disks of transgenic plants, confirmed the higher susceptibility of Swingle citrumelo plants, with high proline production, to this herbicide.
关键词：Citrus paradisi; Poncirus trifoliata; phosphinothricin; P5CS
文献注录：Carneiro CEA, Molinari HBC, Andrade GA, Pereira LFP, Vieira LGE.. Proline production by transgenic plants of Swingle citrumelo and susceptibility to glufosinate ammonium [J]. Pesquisa Agropecuaria Brasileira. 2006, 41(5): 747-753.
报/刊名：《Pesquisa Agropecuaria Brasileira》，发表于2006 / 第 41(5) 期。
文献类型： [J]
页码：第 747-753 页 / 共7 页