作者：Sendin LN, Filippone MP, Orce IG, Rigano L, Enrique R, Pena L, Vojnov AA, Marano MR, Castagnaro AP.
作者单位：Unidad Asociada Inst Super Invest Biol INSIBIO CO, EEAOC
摘要：susceptible host is diminished, indicating that the avrBs2 gene product could confer an adaptive advantage to the pathogen. The avrBs2 of Xanthomonas citri subsp. citri (Xcc), cause of citrus canker, shares 96% homology with avrBs2 of Xcv. To evaluate if Bs2 could recognize avrBs2 of Xcc in citrus plants and thereby activate plant defence mechanisms to increase resistance to canker, transient expression experiments were conducted using Agrobacterium tumefaciens in lemon plants subsequently challenged with wildtype Xcc. The results showed that transient expression of Bs2 reduced canker formation in lemon and induced plant defence mechanisms, as shown by callose deposition and PR-1 expression. Moreover, when an avrBs2 mutant of Xcc was used, no decrease in disease symptoms was observed. This work shows that the Bs2 gene from Solanaceae is functional in lemon, a member of the Rutaceae family. Therefore, Bs2 is a potential candidate gene for stable expression in transgenic citrus plants in order to improve resistance to canker disease.
关键词：avrBs2 avirulence gene; citrus breeding; defence response; disease resistance
文献注录：Sendin LN, Filippone MP, Orce IG, Rigano L, Enrique R, Pena L, Vojnov AA, Marano MR, Castagnaro AP.. Transient expression of pepper Bs2 gene in Citrus limon as an approach to evaluate its utility for management of citrus canker disease [J]. Plant Pathology. 2012, 61(4): 648-657.
报/刊名：《Plant Pathology》，发表于2012 / 第 61(4) 期。
文献类型： [J]
页码：第 648-657 页 / 共10 页